SMAD1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54496
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54496-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54496-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 1356CT119.18.55
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the SMAD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human colon tissue with SMAD1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human skeletal muscle tissue with SMAD1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with SMAD1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Western blot testing of human 1) HT-1080, 2) HeLa and 3) HEK293 cell lysate with SMAD1 antibody. Predicted molecular weight: 52-60 kDa.
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IHC testing of FFPE human colon tissue with SMAD1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human skeletal muscle tissue with SMAD1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with SMAD1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Western blot testing of human 1) HT-1080, 2) HeLa and 3) HEK293 cell lysate with SMAD1 antibody. Predicted molecular weight: 52-60 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the SMAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the SMAD1 antibody.
Limitation:
This SMAD1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
Q15797