ALDH2 Antibody

NSJ Bioreagents
Product Code: NSJ-F40217
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40217-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40217-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, k
Antibody Clonality: Monoclonal
Antibody Clone: 138CT22.3.8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the ALDH2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot analysis of lysate from NCI-H292, A549, HepG2 cell line (left to right) using ALDH2 antibody at 1:1000 for each lane.
2 / 4
ALDH2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue.
3 / 4
ALDH2 antibody flow cytometric analysis of HepG2 cells (red) compared to a negative control cell (blue). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.
4 / 4
Confocal immunofluorescent analysis of ALDH2 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).

Western blot analysis of lysate from NCI-H292, A549, HepG2 cell line (left to right) using ALDH2 antibody at 1:1000 for each lane.
ALDH2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue.
ALDH2 antibody flow cytometric analysis of HepG2 cells (red) compared to a negative control cell (blue). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of ALDH2 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the ALDH2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This ALDH2 antibody was produced from a mouse immunized with ALDH2 recombinant protein.
Limitation:
This ALDH2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P05091