N-Cadherin Antibody / CDH2

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NSJ Bioreagents
Product Code: NSJ-V3390
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3390-20UG20 ug£264.00
Quantity:
NSJ-V3390-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 13A9
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store the N-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human brain lysate with N-Cadherin antibody. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).~
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SDS-PAGE Analysis of Purified, BSA-Free N-Cadherin Antibody (clone 13A9). Confirmation of Int
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Western blot testing of human brain lysate with N-Cadherin antibody. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).~
SDS-PAGE Analysis of Purified, BSA-Free N-Cadherin Antibody (clone 13A9). Confirmation of Int

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the N-Cadherin antibody to be titered up or down for optimal performance.
Description:
Recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CDH2 and CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. NCAD/CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, the protein has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, it is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A human partial recombinant protein corresponding to the CDH2/NCAD cytoplasmic domain was used as the immunogen for this N-Cadherin antibody.
Limitation:
This N-Cadherin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse
Uniprot #:
P19022