PPP2R1B Antibody

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NSJ Bioreagents
Product Code: NSJ-F54528
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54528-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54528-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 1496CT356.164.25.226
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PPP2R1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of 1) human Jurkat, 2) human brain, 3) mouse brain, 4) mouse lung and 5) rat brain lysate with PPP2R1B antibody. Predicted molecular weight ~66 kDa.
2 / 4
IHC testing of FFPE human spleen tissue with PPP2R1B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of fixed and permeabilized human U-2 OS cells with PPP2R1B antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of fixed and permeabilized human Jurkat cells with PPP2R1B antibody; Blue=isotype control, Green= PPP2R1B antibody.
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Western blot testing of 1) human Jurkat, 2) human brain, 3) mouse brain, 4) mouse lung and 5) rat brain lysate with PPP2R1B antibody. Predicted molecular weight ~66 kDa.
IHC testing of FFPE human spleen tissue with PPP2R1B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human U-2 OS cells with PPP2R1B antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of fixed and permeabilized human Jurkat cells with PPP2R1B antibody; Blue=isotype control, Green= PPP2R1B antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the PPP2R1B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the PPP2R1B antibody.
Limitation:
This PPP2R1B antibody is available for research use only.
Purity:
Protein G affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P30154