Aciculin Antibody / Phosphoglucomutase 5

NSJ Bioreagents
Product Code: NSJ-V8411
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8411-20UG20 ug£264.00
Quantity:
NSJ-V8411-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 14F8/F8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Aciculin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
IHC staining of FFPE human uterus with Aciculin antibody (clone 14F8/F8). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human bladder with Aciculin antibody (clone 14F8/F8). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free Aciculin antibody (clone 14F8/F8) as confirmation of integrity and purity.

IHC staining of FFPE human uterus with Aciculin antibody (clone 14F8/F8). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder with Aciculin antibody (clone 14F8/F8). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Aciculin antibody (clone 14F8/F8) as confirmation of integrity and purity.

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the Aciculin antibody should be determined by the researcher.
Description:
Aciculin, also known as PGM5 (phosphoglucomutase 5) or PGMRP, is a 567 amino acid protein that belongs to the phosphoglucomutase family of phosphotransferases, which play an important role in the interconversion of glucose-1-phosphate and glucose-6-phosphate. Localized to the cell junction and expressed at high levels in smooth and cardiac muscle, Aciculin binds magnesium as a cofactor and interacts with dystrophin and utrophin, possibly playing a role in cytoskeletal organization and function. Aciculin exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 9, which houses over 900 genes and comprises nearly 4% of the human genome.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
The cytoskeletal fraction of the human myometrium tissue extract was used as the immunogen for the Aciculin antibody.
Limitation:
This Aciculin antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Q15124