Bid Antibody / BH3-Interacting Domain Death Agonist

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NSJ Bioreagents
Product Code: NSJ-F54478
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54478-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54478-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1,k
Antibody Clonality: Monoclonal
Antibody Clone: 1519CT649.18.47
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the BID antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human bladder tissue with BID antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human 1) Jurkat, 2) A431, 3) ThP-1 and 4) 293T cell lysate with BID antibody. Predicted molecular weight ~22 kDa.
3 / 4
Western blot testing of human 1) Jurkat, 2) A431, 3) MDA-MB-231, 4) Raji, 5) Jurkat and 6) ThP-1 cell lysate with BID antibody. Predicted molecular weight ~22 kDa.
4 / 4
Immunofluorescent staining of fixed and permeabilized human A549 cells with BID antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
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IHC testing of FFPE human bladder tissue with BID antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) Jurkat, 2) A431, 3) ThP-1 and 4) 293T cell lysate with BID antibody. Predicted molecular weight ~22 kDa.
Western blot testing of human 1) Jurkat, 2) A431, 3) MDA-MB-231, 4) Raji, 5) Jurkat and 6) ThP-1 cell lysate with BID antibody. Predicted molecular weight ~22 kDa.
Immunofluorescent staining of fixed and permeabilized human A549 cells with BID antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the BID antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the BID antibody.
Limitation:
This BID antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P55957