ALDH1A1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F40224
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40224-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40224-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 152CT1.2.2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the ALDH1A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from HepG2, K562, NCI-H460 cell line using ALDH1A1 antibody at 1:1000 for each lane.
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Fluorescent confocal image of HepG2 cells stained with ALDH1A1 antibody. ALDH1A1 immunoreactivity is localized to the cytoplasm.
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ALDH1A1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma.
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Confocal immunofluorescent analysis of ALDH1A1 antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).
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Western blot analysis of lysate from HepG2, K562, NCI-H460 cell line using ALDH1A1 antibody at 1:1000 for each lane.
Fluorescent confocal image of HepG2 cells stained with ALDH1A1 antibody. ALDH1A1 immunoreactivity is localized to the cytoplasm.
ALDH1A1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma.
Confocal immunofluorescent analysis of ALDH1A1 antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:100
Application Note:
Titration of the ALDH1A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ALDH1A1 encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This ALDH1A1 antibody was produced from a mouse immunized with ALDH1A1 recombinant protein.
Limitation:
This ALDH1A1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P00352