RAB3A Antibody

NSJ Bioreagents
Product Code: NSJ-F54480
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54480-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54480-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1,k
Antibody Clonality: Monoclonal
Antibody Clone: 1531CT562.14.57
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the RAB3A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human brain lysate with RAB3A antibody. Predicted molecular weight ~25 kDa.
2 / 4
Western blot testing of 1) mouse brain and 2) rat brain lysate with RAB3A antibody. Predicted molecular weight ~25 kDa.
3 / 4
Immunofluorescent staining of fixed and permeabilized rat PC-12 cells with RAB3A antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of fixed and permeabilized rat PC-12 cells with RAB3A antibody; Blue=isotype control, Green= RAB3A antibody.

Western blot testing of human brain lysate with RAB3A antibody. Predicted molecular weight ~25 kDa.
Western blot testing of 1) mouse brain and 2) rat brain lysate with RAB3A antibody. Predicted molecular weight ~25 kDa.
Immunofluorescent staining of fixed and permeabilized rat PC-12 cells with RAB3A antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of fixed and permeabilized rat PC-12 cells with RAB3A antibody; Blue=isotype control, Green= RAB3A antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the RAB3A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the RAB3A antibody.
Limitation:
This RAB3A antibody is available for research use only.
Purity:
Protein G affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P20336