Density Regulated Protein Antibody / DENR

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NSJ Bioreagents
Product Code: NSJ-F54483
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54483-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54483-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b,k
Antibody Clonality: Monoclonal
Antibody Clone: 1542CT106.51.79
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Density Regulated Protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) A431, 2) skeletal muscle and 3) brain lysate with Density Regulated Protein antibody. Expected molecular weight ~22 kDa (unmodified), 27-31 kDa (modified).
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IHC testing of FFPE human skeletal muscle tissue with Density Regulated Protein antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with Density Regulated Protein antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of fixed and permeabilized human HeLa cells with Density Regulated Protein antibody; Blue=isotype control, Green= Density Regulated Protein antibody.
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Western blot testing of human 1) A431, 2) skeletal muscle and 3) brain lysate with Density Regulated Protein antibody. Expected molecular weight ~22 kDa (unmodified), 27-31 kDa (modified).
IHC testing of FFPE human skeletal muscle tissue with Density Regulated Protein antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with Density Regulated Protein antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of fixed and permeabilized human HeLa cells with Density Regulated Protein antibody; Blue=isotype control, Green= Density Regulated Protein antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Density Regulated Protein antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
May be involved in the translation of target mRNAs by scanning and recognition of the initiation codon. Involved in translation initiation; promotes recruitmnet of aminoacetyled initiator tRNA to P site of 40S ribosomes. Can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. Plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the Density Regulated Protein antibody.
Limitation:
This Density Regulated Protein antibody is available for research use only.
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
O43583