TIMP2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54264
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54264-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54264-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 1554CT494.262.47
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TIMP2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human SW480 cell lysate with TIMP2 antibody. Predicted molecular weight ~24 kDa.
2 / 5
Western blot testing of human HT-1080 cell lysate with TIMP2 antibody. Predicted molecular weight ~24 kDa.
3 / 5
Immunofluorescent staining of fixed and permeabilized human A549 cells with TIMP2 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 5
IHC testing of FFPE human kidney tissue with TIMP2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of fixed and permeabilized human K562 cells with TIMP2 antibody; Blue=isotype control, Green= TIMP2 antibody.
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Western blot testing of human SW480 cell lysate with TIMP2 antibody. Predicted molecular weight ~24 kDa.
Western blot testing of human HT-1080 cell lysate with TIMP2 antibody. Predicted molecular weight ~24 kDa.
Immunofluorescent staining of fixed and permeabilized human A549 cells with TIMP2 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human kidney tissue with TIMP2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human K562 cells with TIMP2 antibody; Blue=isotype control, Green= TIMP2 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the TIMP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids from the human protein were used as the immunogen for the TIMP2 Antibody.
Limitation:
This TIMP2 antibody is available for research use only.
Localization:
Cytoplasmic, cell surface, secreted
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P16035