MKRN2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54488
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54488-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54488-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 1556CT631.230.55.49
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the MKRN2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human heart tissue with MKRN2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of fixed and permeabilized human U-2 OS cells with MKRN2 antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of human 1) Jurkat and 2) K562 cell lysate with MKRN2 antibody. Predicted molecular weight ~47 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized human K562 cells with MKRN2 antibody; Blue=isotype control, Green= MKRN2 antibody.
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IHC testing of FFPE human heart tissue with MKRN2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human U-2 OS cells with MKRN2 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human 1) Jurkat and 2) K562 cell lysate with MKRN2 antibody. Predicted molecular weight ~47 kDa.
Flow cytometry testing of fixed and permeabilized human K562 cells with MKRN2 antibody; Blue=isotype control, Green= MKRN2 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the MKRN2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the MKRN2 antibody.
Limitation:
This MKRN2 antibody is available for research use only.
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
Q9H000