Superoxide Dismutase 2 Antibody / SOD2

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NSJ Bioreagents
Product Code: NSJ-F54485
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54485-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54485-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1,kappa
Antibody Clonality: Monoclonal
Antibody Clone: 1559CT572.3.83
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Superoxide Dismutase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human brain tissue with Superoxide Dismutase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human skeletal muscle tissue with Superoxide Dismutase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of 1) human brain, 2) human HeLa, 3) human MCF7, 4) mouse NIH 3T3 and 5) human SH-SY5Y lysate with Superoxide Dismutase 2 antibody. Predicted molecular weight ~25 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized human A549 cells with Superoxide Dismutase 2 antibody; Blue=isotype control, Green= Superoxide Dismutase 2 antibody.
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IHC testing of FFPE human brain tissue with Superoxide Dismutase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human skeletal muscle tissue with Superoxide Dismutase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human brain, 2) human HeLa, 3) human MCF7, 4) mouse NIH 3T3 and 5) human SH-SY5Y lysate with Superoxide Dismutase 2 antibody. Predicted molecular weight ~25 kDa.
Flow cytometry testing of fixed and permeabilized human A549 cells with Superoxide Dismutase 2 antibody; Blue=isotype control, Green= Superoxide Dismutase 2 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Superoxide Dismutase 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the Superoxide Dismutase 2 antibody.
Limitation:
This Superoxide Dismutase 2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity
Species Reactivity :
Human, Mouse
Uniprot #:
P04179