CD44 Antibody / HCAM

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NSJ Bioreagents
Product Code: NSJ-V2068
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2068-20UG20 ug£264.00
Quantity:
NSJ-V2068-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 156-3C11
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the HCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Immunofluorescent staining of human HeLa cells with HCAM antibody (clone 156-3C11, green) and Reddot nuclear stain (red).
2 / 5
IHC testing of FFPE human tonsil stained with HCAM antibody (clone 156-3C11).
3 / 5
IHC testing of FFPE human breast cancer stained with HCAM antibody (clone 156-3C11).
4 / 5
Western blot testing of HeLa cell lysate with HCAM antibody (clone 156-3C11). Predicted molecular weight ~81 kDa.
5 / 5
SDS-PAGE analysis of purified, BSA-free HCAM antibody (clone 156-3C11) as confirmation of integrity and purity.
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Immunofluorescent staining of human HeLa cells with HCAM antibody (clone 156-3C11, green) and Reddot nuclear stain (red).
IHC testing of FFPE human tonsil stained with HCAM antibody (clone 156-3C11).
IHC testing of FFPE human breast cancer stained with HCAM antibody (clone 156-3C11).
Western blot testing of HeLa cell lysate with HCAM antibody (clone 156-3C11). Predicted molecular weight ~81 kDa.
SDS-PAGE analysis of purified, BSA-free HCAM antibody (clone 156-3C11) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 2-4ug/million cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCAM antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes a cell surface glycoprotein of 80-95kDa, called CD44, or HCAM, on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44-s). Higher molecular weight isoforms are described in epithelial cells (CD44-v), which are believed to function in intercellular adhesion and stromal binding. HCAM antibody immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
960
Immunogen:
Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.
Limitation:
This HCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human