VCP Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-F54487
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54487-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54487-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 1563CT163.48.77
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the VCP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human breast cancer tissue with VCP antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with VCP antibody (green) and anti-Actin (red).
3 / 4
Western blot testing of human 1) HeLa, 2) K562, 3) human A549, 4) rat C6, 5) human U-87 MG and 6) mouse NIH 3T3 lysate with VCP antibody. Expected molecular weight: 89-97 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized human K562 cells with VCP antibody; Blue=isotype control, Green= VCP antibody.
❮❯

IHC testing of FFPE human breast cancer tissue with VCP antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with VCP antibody (green) and anti-Actin (red).
Western blot testing of human 1) HeLa, 2) K562, 3) human A549, 4) rat C6, 5) human U-87 MG and 6) mouse NIH 3T3 lysate with VCP antibody. Expected molecular weight: 89-97 kDa.
Flow cytometry testing of fixed and permeabilized human K562 cells with VCP antibody; Blue=isotype control, Green= VCP antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the VCP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the VCP antibody.
Limitation:
This VCP antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Protein G affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P55072