S100A2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54489
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54489-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54489-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 1592CT417.84.81
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the S100A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human skin tissue with S100A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human lung tissue with S100A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with S100A2 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Western blot testing of human 1) A431, 2) HACAT and 3) SW480 cell lysate with S100A2 antibody. Expected molecular weight ~11 kDa.
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IHC testing of FFPE human skin tissue with S100A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human lung tissue with S100A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with S100A2 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Western blot testing of human 1) A431, 2) HACAT and 3) SW480 cell lysate with S100A2 antibody. Expected molecular weight ~11 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the S100A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes. May also play a role in suppressing tumor cell growth.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the S100A2 antibody.
Limitation:
This S100A2 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P29034