Gamma Catenin Antibody / Plakoglobin

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NSJ Bioreagents
Product Code: NSJ-V3205
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3205-20UG20 ug£264.00
Quantity:
NSJ-V3205-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 15F11
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store the Plakoglobin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
Immunofluorescent staining of human HeLa cells with Plakoglobin antibody (clone 15F11, green) and Reddot nuclear stain (red).
2 / 3
Western blot testing of human 1) HT20, 2) HEK293, 3) A431, 4) MCF7, 5) HEPG2 and 6) A549 cell lysate with Plakoglobin antibody (clone 15F11). Predicted molecular weight: 80-87 kDa.
3 / 3
IHC staining of FFPE human cervix with Plakoglobin antibody (clone 15F11). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of human HeLa cells with Plakoglobin antibody (clone 15F11, green) and Reddot nuclear stain (red).
Western blot testing of human 1) HT20, 2) HEK293, 3) A431, 4) MCF7, 5) HEPG2 and 6) A549 cell lysate with Plakoglobin antibody (clone 15F11). Predicted molecular weight: 80-87 kDa.
IHC staining of FFPE human cervix with Plakoglobin antibody (clone 15F11). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Plakoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Plakoglobin, or gamma Catenin, is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of Plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant full length chicken gamma Catenin was used as the immunogen for the Plakoglobin antibody.
Limitation:
This Plakoglobin antibody is available for research use only.
Localization:
Cell surface, cytoplasmic, cell junctions
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P14923