Myosin 18B Antibody / MYO18B

NSJ Bioreagents
Product Code: NSJ-F54495
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54495-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54495-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 1683CT270.39.50
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Myosin 18B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human heart tissue with IRF7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human skeletal muscle tissue with IRF7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of recombinant human partial protein with Myosin 18B antibody.
4 / 4
Flow cytometry testing of fixed and permeabilized human A431 cells with Myosin 18B antibody; Blue=isotype control, Green= Myosin 18B antibody.

IHC testing of FFPE human heart tissue with IRF7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human skeletal muscle tissue with IRF7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of recombinant human partial protein with Myosin 18B antibody.
Flow cytometry testing of fixed and permeabilized human A431 cells with Myosin 18B antibody; Blue=isotype control, Green= Myosin 18B antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Myosin 18B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
May be involved in intracellular trafficking of the muscle cell when in the cytoplasm, whereas entering the nucleus, may be involved in the regulation of muscle specific genes. May play a role in the control of tumor development and progression; restored MYO18B expression in lung cancer cells suppresses anchorage-independent growth.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the Myosin 18B antibody.
Limitation:
This Myosin 18B antibody is available for research use only.
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
Q8IUG5