HLA-DR Antibody (MHC II)

Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml

Optimal dilution of the HLA-DR antibody should be determined by the researcher.

This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Human peripheral blood leukocytes were used as the immunogen for the HLA-DR antibody.

This HLA-DR antibody is available for research use only.

Cell surface

Protein G affinity chromatography

Human

P01903