Anti-Src Antibody

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NSJ Bioreagents
Product Code: NSJ-F40418
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40418-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40418-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 17AT28
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the anti-Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot analysis of lysate from (1) HT29 and (2) Jurkat cell line using anti-Src antibody at 1:1000.
2 / 3
FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src antibody was diluted 1:500 and incubated overnight at 4oC. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
3 / 3
Fluorescent image of A549 cell stained with anti-Src antibody at 1:25. Immunoreactivity is localized to the cytoplasm.
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Western blot analysis of lysate from (1) HT29 and (2) Jurkat cell line using anti-Src antibody at 1:1000.
FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src antibody was diluted 1:500 and incubated overnight at 4oC. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
Fluorescent image of A549 cell stained with anti-Src antibody at 1:25. Immunoreactivity is localized to the cytoplasm.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50,Western blot: 1:100-1000
Application Note:
Titration of the anti-Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-Src kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This anti-Src antibody was produced from a mouse immunized with a full length recombinant human protein.
Limitation:
This anti-Src antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P12931