ICAM-3 Antibody / CD50
Code | Size | Price |
---|
NSJ-V2138-20UG | 20 ug | £264.00 |
Quantity:
NSJ-V2138-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 186-2G9
Regulatory Status: RUO
Target Species: Human
Applications:
- Blocking
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the CD50 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Further Information
Application Details :
Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (order BSA/sodium azide-free format),Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD50 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM3 (Leucocyte Workshop VI). It is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This CD50 antibody is excellent for staining of formalin/paraffin tissues.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
3385
Immunogen:
Stimulated human leukocytes were used as the immunogen for this CD50 antibody.
Limitation:
This CD50 antibody is available for research use only.
Localization:
Membrane, cytoplasm
Purity:
Protein G affinity chromatography
Species Reactivity :
Human