CD45RO Antibody (T-cell marker)

NSJ Bioreagents
Product Code: NSJ-V2416
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2416-20UG20 ug£264.00
Quantity:
NSJ-V2416-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 190-2F2.5
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
The CD45RO antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Images

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Immunofluorescent staining of PFA-fixed human Jurkat cells with CD45RO antibody (clone 190-2F2.5, green) and Reddot nuclear stain (red).~
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Flow cytometry staining of PFA-fixed human Jukat cells with CD45RO antibody (clone 190-2F2.5); Red=

Immunofluorescent staining of PFA-fixed human Jurkat cells with CD45RO antibody (clone 190-2F2.5, green) and Reddot nuclear stain (red).~
Flow cytometry staining of PFA-fixed human Jukat cells with CD45RO antibody (clone 190-2F2.5); Red=

Further Information

Application Details :
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Due to variation in protocol and secondary antibody used, the CD45RO antibody may need to be titered for optimal performance.
Description:
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Lentillectin purified glycoproteins obtained from HuPBL and from a CLL (B cell) were used as immunogen for this CD45RO antibody.
Limitation:
This CD45RO antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P08575