ABO Antibody (Blood Group Antigen H Type 2)

NSJ Bioreagents
Product Code: NSJ-V2548
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2548-20UG20 ug£264.00
Quantity:
NSJ-V2548-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgM, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 19-OLE
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the ABO antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Antigen H Type 2 antibody (19-OLE)

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Antigen H Type 2 antibody (19-OLE)

Further Information

Application Details :
Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the ABO antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min

Description:
Recognizes the blood group H type 2 antigens, trisaccharide Fuc(a1-2)Gal(b1-4)GlcNAc(b1) of human origin. This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Mucinous colonic adenocarcinoma was used as the immunogen for the ABO antibody.
Limitation:
This ABO antibody is available for research use only.
Localization:
Cell surface
Purity:
PEG precipitation
Species Reactivity :
Human
Uniprot #:
P16442