CD31 Antibody

Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1:50-1:100 for 30 min at RT

Optimal dilution of the PECAM antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Bioreactor concentrate

Bioreactor concentrate with 0.05% sodium azide

Prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain downstream of the signal sequence of CD31/PECAM was used as the immunogen for the PECAM antibody.

This PECAM antibody is available for research use only.

Cell surface and cytoplasm of endothelial cells

Unpurified high titer supernatant

Human

P16284