B7-H3 Antibody / CD276

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NSJ Bioreagents
Product Code: NSJ-F54498
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54498-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54498-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 2009CT652.35.9
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the B7-H3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunocytochemical testing of PFA-fixed human 293T cells with B7-H3 antibody. HIER: pH9 EDTA.
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Immunocytochemical testing of PFA-fixed human MCF7 cells with B7-H3 antibody. HIER: pH9 EDTA.
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IHC testing of FFPE human tonsil tissue with B7-H3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Western blot testing of human 1) 293T, 2) A431, 3) U-2 OS, 4) LNCaP, 5) HeLa and 6) MCF7 cell lysate with B7-H3 antibody. Expected molecular weight: 57-110 kDa depending on level of glycosylation.
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Immunocytochemical testing of PFA-fixed human 293T cells with B7-H3 antibody. HIER: pH9 EDTA.
Immunocytochemical testing of PFA-fixed human MCF7 cells with B7-H3 antibody. HIER: pH9 EDTA.
IHC testing of FFPE human tonsil tissue with B7-H3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) 293T, 2) A431, 3) U-2 OS, 4) LNCaP, 5) HeLa and 6) MCF7 cell lysate with B7-H3 antibody. Expected molecular weight: 57-110 kDa depending on level of glycosylation.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25,Immunocytochemistry: 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the B7-H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the B7-H3 antibody.
Limitation:
This B7-H3 antibody is available for research use only.
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
Q5ZPR3