CKB Antibody / Creatine kinase B

ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml for 30 min at RT

Optimal dilution of the Creatine kinase B antibody should be determined by the researcher.

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This mAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Human CKB protein was used as the immunogen for the Creatine kinase B antibody.

This Creatine kinase B antibody is available for research use only.

Cytoplasmic

Protein G affinity chromatography

Human

P12277