IDH2 Antibody / Isocitrate dehydrogenase 2

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NSJ Bioreagents
Product Code: NSJ-RQ6220
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6220-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 2D4
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the IDH2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human A431 cells with IDH2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HeLa, 2) SW620, 3) MCF7, 4) HepG2, 5) Jurkat, 6) rat heart, 7) rat liver, 8) rat PC-12, 9) mouse heart and 10) mouse NIH 3T3 cell lysate with IDH2 antibody. Predicted molecular weight: 45-51 kDa (two isoforms).
3 / 3
Flow cytometry testing of human SiHa cells with IDH2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IDH2 antibody.
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Immunofluorescent staining of FFPE human A431 cells with IDH2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) SW620, 3) MCF7, 4) HepG2, 5) Jurkat, 6) rat heart, 7) rat liver, 8) rat PC-12, 9) mouse heart and 10) mouse NIH 3T3 cell lysate with IDH2 antibody. Predicted molecular weight: 45-51 kDa (two isoforms).
Flow cytometry testing of human SiHa cells with IDH2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IDH2 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the IDH2 antibody should be determined by the researcher.
Description:
Isocitrate dehydrogenase [NADP], mitochondrial is an enzyme that in humans is encoded by the IDH2 gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDR from the human protein were used as the immunogen for the IDH2 antibody.
Limitation:
This IDH2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P48735