Beta-2 Microglobulin Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4629
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4629-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 2H10
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the B2M antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
Immunofluorescent staining of FFPE human A431 cells with B2M antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
IHC staining of FFPE human lung cancer with B2M antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human intestinal cancer with B2M antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human breast cancer with B2M antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of 1) monkey COS-7 and human 2) HeLa, 3) HL-60, 4) HepG2, 5) K562 and 6) 293T lysate with B2M antibody. Predicted molecular weight ~14 kDa.
6 / 6
Flow cytometry testing of human A431 cells with B2M antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B2M antibody.
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Immunofluorescent staining of FFPE human A431 cells with B2M antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung cancer with B2M antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer with B2M antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with B2M antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) monkey COS-7 and human 2) HeLa, 3) HL-60, 4) HepG2, 5) K562 and 6) 293T lysate with B2M antibody. Predicted molecular weight ~14 kDa.
Flow cytometry testing of human A431 cells with B2M antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B2M antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/10^6 cells,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the B2M antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids Q22-M119 from the human protein were used as the immunogen for the B2M antibody.
Limitation:
This B2M antibody is available for research use only.
Purity:
Protein G affinity
Species Reactivity :
Human, Monkey
Uniprot #:
P61769