HER2 Antibody / ErbB2

NSJ Bioreagents
Product Code: NSJ-V7586
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7586-20UG20 ug£264.00
Quantity:
NSJ-V7586-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 2KRI7-3
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human breast cancer with HER2 antibody (clone 2KRI7-3). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human breast cancer with HER2 antibody (clone 2KRI7-3). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Optimal dilution of the HER2 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
HER2/ErbB2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary. [RefSeq]
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human HER2 protein fragment within amino acids 311-462 was used as the immunogen for the HER2 antibody.
Limitation:
This HER2 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P04626