RIZ1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F40202
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40202-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40202-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 33AT1045
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • ChIP
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot the RIZ1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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ChIP analysis: soluble chromatin was prepared from MCF7 cells treated/untreated with E2 for 45 min. Immunoprecipitation was performed with RIZ1 antibody. DNA extractions amplified using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter.
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Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed with RIZ1 antibody and control immunoglobulin G as indicated.
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ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts with RIZ1 antibody. Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the Cdc25c promoter region.
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Western blot analysis of PRDM2 / RIZ1 antibody and transfected cell lysate
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ChIP analysis: soluble chromatin was prepared from MCF7 cells treated/untreated with E2 for 45 min. Immunoprecipitation was performed with RIZ1 antibody. DNA extractions amplified using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter.
Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed with RIZ1 antibody and control immunoglobulin G as indicated.
ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts with RIZ1 antibody. Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the Cdc25c promoter region.
Western blot analysis of PRDM2 / RIZ1 antibody and transfected cell lysate

Documents

Further Information

Application Details :
Western blot: 1:1000,ChIP: 1:100
Application Note:
Titration of the RIZ1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Purified recombinant GST fusion protein encoding aa 1-347 of human RIZ1. This RIZ1 antibody is specific and does not recognize the beta isoform (RIZ2).
Limitation:
This RIZ1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q13029