UCHL1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F40308
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40308-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40308-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, k
Antibody Clonality: Monoclonal
Antibody Clone: 346CT2.2.1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the UCHL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from 1) NCI-H460, 2) U266, 3) mouse Neuro-2a, rat 4) PC-12 and 5) C6 cell line and 6) mouse brain tissue using UCHL1 antibody at 1:1000. Predicted molecular weight ~25 kDa.
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Immunohistochemical analysis of paraffin-embedded human kidney using UCHL1 at 1:25 dilution.
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Fluorescent image of A549 cells stained with UCHL1 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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Western blot analysis of lysate from 1) NCI-H460, 2) U266, 3) mouse Neuro-2a, rat 4) PC-12 and 5) C6 cell line and 6) mouse brain tissue using UCHL1 antibody at 1:1000. Predicted molecular weight ~25 kDa.
Immunohistochemical analysis of paraffin-embedded human kidney using UCHL1 at 1:25 dilution.
Fluorescent image of A549 cells stained with UCHL1 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Documents

Further Information

Application Details :
IHC (Paraffin): 1:25,Immunofluorescence: 1:25,Western blot: 1:120-1:1000
Application Note:
Titration of the UCHL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This UCHL1 antibody was produced from a mouse immunized with UCHL1 recombinant protein.
Limitation:
This UCHL1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P09936