ZO-1 Antibody / TJP1

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NSJ Bioreagents
Product Code: NSJ-RQ5546
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5546-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3E12.
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TJP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human kidney cancer with TJP1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human placenta with TJP1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 5
Flow cytometry testing of human HeLa cells with TJP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TJP1 antibody.
4 / 5
Immunofluorescent staining of FFPE human MCF7 cells with TJP1 antibody (green) and DAPI nuclear stain (blue).
5 / 5
Western blot testing of human 1) Caco-2, 2) U-2 OS, 3) K562, 4) PC-3 and 5) T-47D lysate with TJP1 antibody. Predicted molecular weight ~187 kDa.
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IHC staining of FFPE human kidney cancer with TJP1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human placenta with TJP1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human HeLa cells with TJP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TJP1 antibody.
Immunofluorescent staining of FFPE human MCF7 cells with TJP1 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human 1) Caco-2, 2) U-2 OS, 3) K562, 4) PC-3 and 5) T-47D lysate with TJP1 antibody. Predicted molecular weight ~187 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the TJP1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Tight junction protein ZO-1 is a protein that in humans is encoded by the TJP1 gene. It is mapped to 15q13.1. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. It has been found that injected CagA associates with the epithelial tight-junction scaffolding protein TJP1 and the transmembrane protein junctional adhesion molecule, causing an ectopic assembly of tight junction components at sites of bacterial attachment, and altering the composition and function of the apical-junctional complex.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids H1178-F1527) was used as the immunogen for the TJP1 antibody.
Limitation:
This TJP1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q07157