von Willebrand Factor Antibody / vWF

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NSJ Bioreagents
Product Code: NSJ-V2298
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2298-20UG20 ug£264.00
Quantity:
NSJ-V2298-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 3E2D10
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the vWF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of human tonsil with vWF antibody (clone 3E2D10). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2 / 3
IHC testing of FFPE human pancreas tissue with vWF antibody (clone 3E2D10). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
3 / 3
Immunofluorescent staining of human brain tissue with vWF antibody (clone 3E2D10, green) and DAPI nuclear stain (blue).
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IHC staining of human tonsil with vWF antibody (clone 3E2D10). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
IHC testing of FFPE human pancreas tissue with vWF antibody (clone 3E2D10). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
Immunofluorescent staining of human brain tissue with vWF antibody (clone 3E2D10, green) and DAPI nuclear stain (blue).

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the vWF antibody to be titered up or down for optimal performance.
Description:
von Willebrand Factor is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This vWF antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi�s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
7450
Immunogen:
Amino acids 845-949 were used as the immunogen for this vWF antibody.
Limitation:
This vWF antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G purified vWF antibody
Species Reactivity :
Human