eRF1 Antibody / EFT1

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NSJ Bioreagents
Product Code: NSJ-RQ6232
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6232-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 3E5.
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ETF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 11
Western blot testing of rat 1) liver, 2) testis, 3) kidney, 4) PC-12, and mouse 5) liver, 6) testis, 7) kidney and 8) RAW264.7 cell lysate with ETF1 antibody. Predicted molecular weight ~49 kDa.
2 / 11
Western blot testing of human 1) HeLa, 2) Jurkat, 3) K562, 4) Raji and 5) HepG2 cell lysate with ETF1 antibody. Predicted molecular weight ~49 kDa.
3 / 11
Immunofluorescent staining of FFPE human A431 cells with ETF1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 11
IHC staining of FFPE human lung cancer with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 11
IHC staining of FFPE human breast cancer with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human tonsil with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE human liver cancer with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 11
IHC staining of FFPE rat pancreas with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 11
Flow cytometry testing of human Caco-2 cells with ETF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ETF1 antibody.
10 / 11
Flow cytometry testing of mouse HEPA1-6 cells with ETF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ETF1 antibody.
11 / 11
Flow cytometry testing of rat RH35 cells with ETF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ETF1 antibody.
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Western blot testing of rat 1) liver, 2) testis, 3) kidney, 4) PC-12, and mouse 5) liver, 6) testis, 7) kidney and 8) RAW264.7 cell lysate with ETF1 antibody. Predicted molecular weight ~49 kDa.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) K562, 4) Raji and 5) HepG2 cell lysate with ETF1 antibody. Predicted molecular weight ~49 kDa.
Immunofluorescent staining of FFPE human A431 cells with ETF1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung cancer with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat pancreas with EFT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human Caco-2 cells with ETF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ETF1 antibody.
Flow cytometry testing of mouse HEPA1-6 cells with ETF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ETF1 antibody.
Flow cytometry testing of rat RH35 cells with ETF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ETF1 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the ETF1 antibody should be determined by the researcher.
Description:
Eukaryotic translation termination factor 1 (eRF1), also known as TB3-1, is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids D9-K342) was used as the immunogen for the ETF1 antibody.
Limitation:
This ETF1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P62495