RAB5A Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4897
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4897-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 3E9.
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the RAB5A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) placenta, 3) A549, 4) A375, 5) SW620, 6) U-2 OS, 7) HEK293, 8) U-87 MG, 9) rat brain, 10) mouse brain and 11) mouse Neuro-2a lysate with RAB5A antibody. Predicted molecular weight ~25 kDa.
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Immunofluorescent staining of FFPE human MCF7 cells with RAB5A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human A431 cells with RAB5A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB5A antibody.
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Western blot testing of human 1) HeLa, 2) placenta, 3) A549, 4) A375, 5) SW620, 6) U-2 OS, 7) HEK293, 8) U-87 MG, 9) rat brain, 10) mouse brain and 11) mouse Neuro-2a lysate with RAB5A antibody. Predicted molecular weight ~25 kDa.
Immunofluorescent staining of FFPE human MCF7 cells with RAB5A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A431 cells with RAB5A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB5A antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Flow Cytometry: 1-3ug/10^6 cells,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the RAB5A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP).RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN from the human protein were used as the immunogen for the RAB5A antibody.
Limitation:
This RAB5A antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P20339