Ubiquitin-protein ligase E3A Antibody / UBE3A

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NSJ Bioreagents
Product Code: NSJ-RQ6275
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6275-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 3F13
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the UBE3A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U-87 MG cells with UBE3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBE3A antibody.
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Western blot testing of human 1) Jurkat, 2) HEK293, 3) Raji and 4) monkey COS-7 cell lysate with UBE3A antibody. Predicted molecular weight ~100 kDa.
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Flow cytometry testing of human U-87 MG cells with UBE3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBE3A antibody.
Western blot testing of human 1) Jurkat, 2) HEK293, 3) Raji and 4) monkey COS-7 cell lysate with UBE3A antibody. Predicted molecular weight ~100 kDa.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the UBE3A antibody should be determined by the researcher.
Description:
Ubiquitin-protein ligase E3A (UBE3A) also known as E6AP ubiquitin-protein ligase (E6AP) is an enzyme that in humans is encoded by the UBE3A gene. It is mapped to 15q11.2. This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids M1-E860) was used as the immunogen for the UBE3A antibody.
Limitation:
This UBE3A antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Monkey
Uniprot #:
Q05086