PTBP1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5657
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5657-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 3H12
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PTBP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human tonsil with PTBP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE rat intestine with PTBP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 8
IHC staining of FFPE human rectal cancer with PTBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse intestine with PTBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat intestine with PTBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
Immunofluorescent staining of FFPE human HeLa cells with PTBP1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 8
Western blot testing of human 1) HeLa, 2) K562, 3) A549, 4) HEK293, 5) Jurkat, 6) Raji, 7) rat PC-12 and 8) mouse NIH 3T3 lysate with PTBP1 antibody. Predicted molecular weight ~57 kDa.
8 / 8
Flow cytometry testing of human K562 cells with PTBP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PTBP1 antibody.
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Immunofluorescent staining of FFPE human tonsil with PTBP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat intestine with PTBP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human rectal cancer with PTBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine with PTBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with PTBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with PTBP1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) K562, 3) A549, 4) HEK293, 5) Jurkat, 6) Raji, 7) rat PC-12 and 8) mouse NIH 3T3 lysate with PTBP1 antibody. Predicted molecular weight ~57 kDa.
Flow cytometry testing of human K562 cells with PTBP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PTBP1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the PTBP1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Polypyrimidine tract-binding protein 1 is a protein that in humans is encoded by the PTBP1 gene. It is mapped to 19p13.3. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-A504) was used as the immunogen for the PTBP1 antibody.
Limitation:
This PTBP1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P26599