CD137 Antibody / 4-1BB / TNFRSF9

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NSJ Bioreagents
Product Code: NSJ-V7567
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7567-20UG20 ug£264.00
Quantity:
NSJ-V7567-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 4-1BB/3201
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the CD137 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 7
Immunofluorescence staining of MeOH-fixed human HEK293 cells with CD137 antibody (green, clone 4-1BB/3201) and Reddot nuclear stain (red).
2 / 7
IHC staining of FFPE human spleen with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human renal cell carcinoma with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human renal cell carcinoma with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
5 / 7
Flow cytometry testing of MeOH-fixed human HEK293 cells with CD137 antibody (clone 4-1BB/3201); Red=isotype control, Blue= CD137 mAb.
6 / 7
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD137 antibody (clone 4-1BB/3201). These results demonstrate the foremost specificity of the 4-1BB/3201 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
7 / 7
Western blot testing of human kidney lysate with CD137 antibody. Expected molecular weight: ~30 kDa monomer and 55-60 kDa dimer. Higher molecular weights may be observed due to glycosylation.
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Immunofluorescence staining of MeOH-fixed human HEK293 cells with CD137 antibody (green, clone 4-1BB/3201) and Reddot nuclear stain (red).
IHC staining of FFPE human spleen with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human renal cell carcinoma with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human renal cell carcinoma with CD137 antibody (clone 4-1BB/3201). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Flow cytometry testing of MeOH-fixed human HEK293 cells with CD137 antibody (clone 4-1BB/3201); Red=isotype control, Blue= CD137 mAb.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD137 antibody (clone 4-1BB/3201). These results demonstrate the foremost specificity of the 4-1BB/3201 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Western blot testing of human kidney lysate with CD137 antibody. Expected molecular weight: ~30 kDa monomer and 55-60 kDa dimer. Higher molecular weights may be observed due to glycosylation.

Documents

Further Information

Application Details :
ELISA (order BSA/sodium azide-free format for coating),Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the CD137 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
CD137 belongs to the tumor necrosis factor receptor family and delivers a costimulatory signal to T lymphocytes. It is expressed on activated T cells and binds an inducible ligand that is found on B cells, macrophages and dendritic cells. Interactions between CD137 and its ligand are involved in antigen presentation and the generation of cytotoxic T cells. CD137 antibody may improve cancer treatment, and has been implicated in breast cancer, melanoma and lymphoma.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A recombinant human CD137 protein fragment within amino acids 19-188 was used as the immunogen for the CD137 antibody.
Limitation:
This CD137 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Q07011