PSMA5 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54524
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54524-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54524-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 426CT8.5.1
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PSMA5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) liver, 2) HeLa, 3) HepG2, 4) MCF7, 5) mouse Neuro-2a and 6) rat PC-12 cell lysate with PSMA5 antibody. Predicted molecular weight ~26 kDa.
2 / 4
Western blot testing of human K562 cell lysate with PSMA5 antibody. Predicted molecular weight ~26 kDa.
3 / 4
Immunofluorescent staining of human HeLa cells with PSMA5 antibody (green) and anti-Actin (red).
4 / 4
IHC testing of FFPE human testis tissue with PSMA5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) liver, 2) HeLa, 3) HepG2, 4) MCF7, 5) mouse Neuro-2a and 6) rat PC-12 cell lysate with PSMA5 antibody. Predicted molecular weight ~26 kDa.
Western blot testing of human K562 cell lysate with PSMA5 antibody. Predicted molecular weight ~26 kDa.
Immunofluorescent staining of human HeLa cells with PSMA5 antibody (green) and anti-Actin (red).
IHC testing of FFPE human testis tissue with PSMA5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the PSMA5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the PSMA5 antibody.
Limitation:
This PSMA5 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Protein G affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P28066