SET07 Antibody

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NSJ Bioreagents
Product Code: NSJ-F40198
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40198-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40198-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 43AT551.86
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SET07 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of 293 lysate using SET07 antibody at 1:1000.
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IHC analysis of FFPE human testis tissue stained with SET07 antibody
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Immunofluorescence analysis of SET07 antibody and HeLa cells with (bottom) and without (top) PE-conjugated goat anti-mouse lgG secondary.
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Western blot analysis of 293 lysate using SET07 antibody at 1:1000.
IHC analysis of FFPE human testis tissue stained with SET07 antibody
Immunofluorescence analysis of SET07 antibody and HeLa cells with (bottom) and without (top) PE-conjugated goat anti-mouse lgG secondary.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the SET07 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SET07 is a protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This SET07 antibody was raised using purified recombinant GST fusion protein encoding the N-terminal region of human SET07.
Limitation:
This SET07 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9NQR1