ATG12 Antibody

NSJ Bioreagents
Product Code: NSJ-F40210
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40210-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40210-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, k
Antibody Clonality: Monoclonal
Antibody Clone: 43CT73.3.5.5.4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the ATG12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of ATG12 antibody and recombinant protein.
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IHC analysis of FFPE human hepatocarcinoma tissue and lung carcinoma tissue stained with ATG12 antibody
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IHC analysis of FFPE human lung carcinoma tissue stained with ATG12 antibody
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ATG12 antibody tested in 293 cells, fixed in PFA permeablilized with 0.2% Saponin, blocked with 10% goat serum. Secondary was mouse 555. The right is in full medium (FM) and the left with EBSS (no Leupeptin) (Provided by Nicole McKnight & Sharon Tooze).

Western blot analysis of ATG12 antibody and recombinant protein.
IHC analysis of FFPE human hepatocarcinoma tissue and lung carcinoma tissue stained with ATG12 antibody
IHC analysis of FFPE human lung carcinoma tissue stained with ATG12 antibody
ATG12 antibody tested in 293 cells, fixed in PFA permeablilized with 0.2% Saponin, blocked with 10% goat serum. Secondary was mouse 555. The right is in full medium (FM) and the left with EBSS (no Leupeptin) (Provided by Nicole McKnight & Sharon Tooze).

Further Information

Application Details :
Western blot: 1:200-1:2000,IHC (Paraffin): 1:50-1:200,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the ATG12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
APG12L recombinant protein was used to produce this monoclonal antibody.
Limitation:
This ATG12 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
O94817