EPHB2 Antibody

NSJ Bioreagents
Product Code: NSJ-F40413
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40413-0.05ML0.05 ml£260.00
Quantity:
NSJ-F40413-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, k
Antibody Clonality: Monoclonal
Antibody Clone: 48CT12.6.4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the EPHB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis partial EPHB2 protein using EphB2 antibody at 1:1000.
2 / 3
Flow cytometric analysis of HepG2 cells using EPHB2 antibody (red) compared to a negative control (blue). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.
3 / 3
IHC analysis of FFPE human lung carcinoma tissue stained with EPHB2 antibody

Western blot analysis partial EPHB2 protein using EphB2 antibody at 1:1000.
Flow cytometric analysis of HepG2 cells using EPHB2 antibody (red) compared to a negative control (blue). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.
IHC analysis of FFPE human lung carcinoma tissue stained with EPHB2 antibody

Further Information

Application Details :
Western blot: 1:1000,Flow Cytometry: 1:10-1:50,IHC (Paraffin): 1:200
Application Note:
Titration of the EPHB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Purified His-tagged protein (from amino acids 127~425) was used to produce this monoclonal EPHB2 antibody.
Limitation:
This EPHB2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P29323