MET Antibody (HGFR)

NSJ Bioreagents
Product Code: NSJ-F40179
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40179-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40179-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 4AT44
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the MET antibody (HGFR) and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Immunohistochemical analysis of paraffin-embedded human liver using MET antibody at 1:25 dilution.
2 / 4
Western blot analysis of lysate from HeLa, HepG2, COS-7 cell line (left to right) using MET antibody at 1:1000 for each lane.
3 / 4
Fluorescent confocal image of HepG2 cells stained with MET antibody. MET is localized to the cytoplasm.
4 / 4
IHC analysis of FFPE human colon carcinoma tissue stained with MET antibody

Immunohistochemical analysis of paraffin-embedded human liver using MET antibody at 1:25 dilution.
Western blot analysis of lysate from HeLa, HepG2, COS-7 cell line (left to right) using MET antibody at 1:1000 for each lane.
Fluorescent confocal image of HepG2 cells stained with MET antibody. MET is localized to the cytoplasm.
IHC analysis of FFPE human colon carcinoma tissue stained with MET antibody

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:100,IHC (Paraffin): 1:25-1:100
Application Note:
Titration of the MET antibody (HGFR) may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This MET antibody was produced from mice immunized with purified recombinant protein encoding the catalytic domain of human MET/HGFR.
Limitation:
This MET antibody (HGFR) is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P08581