CA1 Antibody / Carbonic Anhydrase I

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NSJ Bioreagents
Product Code: NSJ-RQ6239
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6239-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 4D5
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Carbonic Anhydrase I antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human SiHa cells with Carbonic Anhydrase I antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Carbonic Anhydrase I antibody.
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IHC staining of FFPE human placenta with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human rectal cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE human breast cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human liver cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human esophageal squamous carcinoma with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Western blot testing of 1) human K562, 2) rat spleen and 3) mouse spleen lysate with Carbonic Anhydrase I antibody. Predicted molecular weight: ~29 kDa.
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Flow cytometry testing of human SiHa cells with Carbonic Anhydrase I antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Carbonic Anhydrase I antibody.
IHC staining of FFPE human placenta with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human K562, 2) rat spleen and 3) mouse spleen lysate with Carbonic Anhydrase I antibody. Predicted molecular weight: ~29 kDa.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Carbonic Anhydrase I antibody should be determined by the researcher.
Description:
Carbonic anhydrase 1 is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participates in a variety of biologic processes like respiration, calcification, acid-base balance etc.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids D9-F261) was used as the immunogen for the Carbonic Anhydrase I antibody.
Limitation:
This Carbonic Anhydrase I antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P00915