Cystatin C Antibody / Cystatin 3

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NSJ Bioreagents
Product Code: NSJ-RQ4885
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4885-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 4H8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Capture Antibody
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cystatin C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of human 1) PC-3, 2) Caco-2, 3) placenta, 4) A549, 5) COLO-320 and 6) HEK293 lysate with Cystatin C antibody. Predicted molecular weight ~16 kDa.
2 / 6
IHC staining of FFPE human glioma with Cystatin C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human colon cancer with Cystatin C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human colon cancer with Cystatin C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Immunofluorescent staining of FFPE human MCF7 cells with Cystatin C antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
6 / 6
Flow cytometry testing of human A549 cells with Cystatin C antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cystatin C antibody.
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Western blot testing of human 1) PC-3, 2) Caco-2, 3) placenta, 4) A549, 5) COLO-320 and 6) HEK293 lysate with Cystatin C antibody. Predicted molecular weight ~16 kDa.
IHC staining of FFPE human glioma with Cystatin C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer with Cystatin C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer with Cystatin C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with Cystatin C antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with Cystatin C antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cystatin C antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml, Flow Cytometry: 1-3ug/10^6 cells,Immunofluorescence (FFPE): 2-4ug/ml,ELISA (Capture): 1-5ug/ml
Application Note:
Optimal dilution of the Cystatin C antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Cystatin C or cystatin 3, a protein encoded by the CST3 gene, is mainly used as a biomarker of kidney function. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer's disease. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and body fluids. It is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Cystatin C belongs to the type 2 cystatin gene family.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids K31-A146 from the human protein were used as the immunogen for the Cystatin C antibody.
Limitation:
This Cystatin C antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P01034