EGFR Antibody

NSJ Bioreagents
Product Code: NSJ-F40415
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40415-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40415-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, k
Antibody Clonality: Monoclonal
Antibody Clone: 51CT78.40.5
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the EGFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot analysis of lysate from A431, MDA-MB-468 cell line (left to right) using EGFR antibody at 1:1000 for each lane.
2 / 3
IHC analysis of FFPE human breast carcinoma with EGFR antibody
3 / 3
Flow cytometric analysis of HepG2 cells using EGFR antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.

Western blot analysis of lysate from A431, MDA-MB-468 cell line (left to right) using EGFR antibody at 1:1000 for each lane.
IHC analysis of FFPE human breast carcinoma with EGFR antibody
Flow cytometric analysis of HepG2 cells using EGFR antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:2000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the EGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Purified His-tagged protein fragment was used to produced this monoclonal EGFR antibody.
Limitation:
This EGFR antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P00533