LIN28A Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-F40207
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40207-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40207-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, k
Antibody Clonality: Monoclonal
Antibody Clone: 55CT58.12.1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the LIN28A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot analysis of recombinant protein with LIN28A antibody
2 / 3
IHC analysis of FFPE human testis tissue stained with LIN28A antibody
3 / 3
Immunofluorescence analysis of LIN28A antibody with paraffin-embedded human testis tissue. Primary antibody was followed by PE-conjugated goat anti-mouse lgG. PE emits orange fluorescence.
❮❯

Western blot analysis of recombinant protein with LIN28A antibody
IHC analysis of FFPE human testis tissue stained with LIN28A antibody
Immunofluorescence analysis of LIN28A antibody with paraffin-embedded human testis tissue. Primary antibody was followed by PE-conjugated goat anti-mouse lgG. PE emits orange fluorescence.

Documents

Further Information

Application Details :
Western blot: 1:200-1:2000,IHC (Paraffin): 1:50-1:200,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the LIN28A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
LIN28A is a translational enhancer that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits ZCCHC11/TUT4 uridylyltransferase. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation.[UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
LIN28A recombinant protein was used to produce this monoclonal antibody.
Limitation:
This LIN28A antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q9H9Z2