KIT Antibody

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NSJ Bioreagents
Product Code: NSJ-F40394
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40394-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40394-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgM
Antibody Clonality: Monoclonal
Antibody Clone: 566CT8.5.4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the KIT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunofluorescent analysis of U251 cells using KIT antibody. Ab was diluted at 1:100 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
2 / 3
Fluorescent confocal image of SY5Y cells stained with KIT antibody at 1:50. Note the highly specific localization of the immunosignal to the membranes, especially the plasma membrane.
3 / 3
KIT antibody western blot analysis in A549 lysate
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Immunofluorescent analysis of U251 cells using KIT antibody. Ab was diluted at 1:100 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Fluorescent confocal image of SY5Y cells stained with KIT antibody at 1:50. Note the highly specific localization of the immunosignal to the membranes, especially the plasma membrane.
KIT antibody western blot analysis in A549 lysate

Documents

Further Information

Application Details :
Western blot: 1:100,Immunofluorescence: 1:10-1:100
Application Note:
Titration of the KIT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This KIT antibody was produced from mice immunized with human KIT recombinant protein.
Limitation:
This KIT antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P10721