KLF4 Antibody

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NSJ Bioreagents
Product Code: NSJ-F40406
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40406-0.05ML0.05 ml£260.00
Quantity:
NSJ-F40406-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 56CT5.1.6
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the KLF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Immunofluorescence analysis with KLF4 antibody. HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.
2 / 3
Flow cytometric analysis of MCF-7 cells using KLF4 antibody (bottom histogram) compared to a negative control (top histogram). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.
3 / 3
Western blot analysis of recombinant protein using KLF4 antibody at 1:1000.
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Immunofluorescence analysis with KLF4 antibody. HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.
Flow cytometric analysis of MCF-7 cells using KLF4 antibody (bottom histogram) compared to a negative control (top histogram). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.
Western blot analysis of recombinant protein using KLF4 antibody at 1:1000.

Documents

Further Information

Application Details :
Western blot: 1:2000,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the KLF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
KLF4 is a transcription factor that can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant protein was used to produce this monoclonal KLF4 antibody.
Limitation:
This KLF4 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
O43474