Estrogen Receptor beta 2 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-V8668
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8668-20UG20 ug£264.00
Quantity:
NSJ-V8668-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 57/3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Estrogen Receptor beta 2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
Flow cytometry testing of PFA-fixed human MCF7 cells with Estrogen Receptor beta 2 antibody (clone 57/3); Red=isotype control, Blue= Estrogen Receptor beta 2 antibody.
2 / 3
IHC staining of FFPE human testis with Estrogen Receptor beta 2 antibody (clone 57/3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free Estrogen Receptor beta 2 antibody (clone 57/3) as confirmation of integrity and purity.
❮❯

Flow cytometry testing of PFA-fixed human MCF7 cells with Estrogen Receptor beta 2 antibody (clone 57/3); Red=isotype control, Blue= Estrogen Receptor beta 2 antibody.
IHC staining of FFPE human testis with Estrogen Receptor beta 2 antibody (clone 57/3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Estrogen Receptor beta 2 antibody (clone 57/3) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 2-4ug/million cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the Estrogen Receptor beta 2 antibody should be determined by the researcher.
Description:
This MAb recognizes the estrogen receptor beta 2 isoform, expressed in cell nuclei of a range of human tissues including ovary, placenta, testis and vas deferens. No cross-reactivity is seen with estrogen receptor beta 1 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Amino acids CGMKMETLLPEATMEQ from the C terminus of the human estrogen receptor beta 2 isomer were used as the immunogen for the Estrogen Receptor beta 2 antibody.
Limitation:
This Estrogen Receptor beta 2 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Q92731