SOX2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F40361
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40361-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40361-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 57CT23.3.4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SOX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from NCCIT cell line using SOX2 antibody at 1:1000.
2 / 6
Western blot analysis of SOX2 recombinant protein using SOX2 antibody at 1:4000.
3 / 6
Fluorescent image of A549 cell stained with SOX2 antibody at 1:25. Immunoreactivity is localized to the nucleus.
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Western blot analysis of SOX2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (2)
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IHC analysis of FFPE human lung carcinoma tissue stained with SOX2 antibody
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Flow cytometric analysis of NCI-H460 cells using SOX2 antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.
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Western blot analysis of lysate from NCCIT cell line using SOX2 antibody at 1:1000.
Western blot analysis of SOX2 recombinant protein using SOX2 antibody at 1:4000.
Fluorescent image of A549 cell stained with SOX2 antibody at 1:25. Immunoreactivity is localized to the nucleus.
Western blot analysis of SOX2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (2)
IHC analysis of FFPE human lung carcinoma tissue stained with SOX2 antibody
Flow cytometric analysis of NCI-H460 cells using SOX2 antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50,Western blot: 1:200-1:2000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the SOX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
recombinant protein was used to produce this monoclonal SOX2 antibody.
Limitation:
This SOX2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P48431